Microfluidic multi-analyte gradient generator

Universal microfluidic gradient generator.

The study of cellular responses to chemical gradients in vitro would greatly benefit from experimental systems that can generate precise and stable gradients comparable to chemical nonhomogeneities occurring in vivo. Recently, microfluidic devices have been demonstrated for linear gradient generation for biological applications with unmatched accuracy and stability. However, no systematic appro...

Microfluidic multiplexed multi-analyte immunosensing platform

In this work we present an electrochemical microfluidic platform for the detection of multiple analytes. The developed system allows the simultaneous readout of up to four different enzyme-linked assays even in various formats. The applicability was shown with the antibiotic detection of tetracycline in different concentrations by means of a DNA-based assay on a single chip. With a sample-to-re...

Multi-analyte cellular assays in a microfluidic dipstick

A Transwell Microfluidic Gradient Generator for Cell Culture

We describe and characterize a microfluidic device that enables the generation of quantitative biomolecular gradients in an innovative format which interfaces directly with pre-existing cell cultures. Our device is a simple, easy-to-use add-on that utilizes conventional 6-well plates and the format of Transwell inserts to support a microfluidic membrane-based gradient generator that is untether...

Adaptive microfluidic gradient generator for quantitative chemotaxis experiments.

Chemotactic motion in a chemical gradient is an essential cellular function that controls many processes in the living world. For a better understanding and more detailed modelling of the underlying mechanisms of chemotaxis, quantitative investigations in controlled environments are needed. We developed a setup that allows us to separately address the dependencies of the chemotactic motion on t...